Fourth-generation markerless microscopic imaging technology

Intensity diffraction tomography (IDT) utilizes the intracellular refractive index as an "endogenous dye" to establish a quantitative correlation between the intensity stack and the three-dimensional refractive index distribution of the object. It employs annular matched illumination to optimize the three-dimensional phase transfer function, and reconstructs the three-dimensional refractive index distribution of cells from the recorded intensity images through a related four-dimensional optical transfer function deconvolution algorithm. IDT technology not only extends the imaging resolution of three-dimensional diffraction tomography to the incoherent diffraction limit but also provides high contrast, anti-scattering, and high axial tomographic three-dimensional imaging capabilities for complex samples, offering a label-free quantitative analysis method for the imaging and analysis of subcellular structures in living cells.

Development history of unlabeled microscopic imaging technology

IDT
Phase Contrast
QPI
ODT

1934

Frits Zernike
IDT
Phase Contrast
QPI
ODT

1948~

Dennis Gabor
IDT
Phase Contrast
QPI
ODT

1969~

Emil Wolf
IDT
Phase Contrast
QPI
ODT

2012~

锆石光电

What is the light intensity diffraction tomography technology

Light intensity diffraction tomography technology enables high-resolution three-dimensional imaging of cells and their organelles, making it a high-content imaging technique that is highly suitable for basic in vitro research and drug development. Furthermore, this label-free method is non-invasive and does not produce harmful effects such as phototoxicity and chemical dyes. This means that cells can be imaged for several consecutive days without being damaged, enabling the study of long-term processes such as stem cell differentiation.

Light intensity diffraction tomography technology enables high-resolution three-dimensional imaging of cells and their organelles, making it a high-content imaging technique that is highly suitable for basic in vitro research and drug development. Furthermore, this label-free method is non-invasive and does not produce harmful effects such as phototoxicity and chemical dyes. This means that cells can be imaged for several consecutive days without being damaged, enabling the study of long-term processes such as stem cell differentiation.

Light intensity diffraction tomography technology enables high-resolution three-dimensional imaging of cells and their organelles, making it a high-content imaging technique that is highly suitable for basic in vitro research and drug development. Furthermore, this label-free method is non-invasive and does not produce harmful effects such as phototoxicity and chemical dyes. This means that cells can be imaged for several consecutive days without being damaged, enabling the study of long-term processes such as stem cell differentiation.

Light intensity transmission theory

By measuring the light intensity distribution at different focal planes, the phase information of the sample is reconstructed using the light intensity transmission equation, and then the three-dimensional refractive index distribution is reconstructed

Synthetic aperture technology

Combining the concept of multi angle illumination, a three-dimensional light intensity data stack is obtained through a circular illumination system, without the need for coherent illumination and interferometric measurement

Frequency domain filling algorithm

By using deconvolution of the three-dimensional phase transfer function, the three-dimensional refractive index distribution of an object can be directly reconstructed, breaking through the dependence of traditional optical diffraction tomography techniques on interferometry and beam mechanical scanning

What is the unique method of intensity diffraction tomography for zircon photoelectrons?

Programmable LED Light Source

Illumination Angle Scanning

Two-Dimensional Projections of Cell CT

Two-Dimensional Spectrum of Cell CT

Three-Dimensional Spectral Reconstruction

Three-Dimensional Refractive Index Reconstruction

Why does the intensity diffraction tomography of zircon achieve such high resolution?

Using the internal refractive index of cells as the "endogenous dye", a quantitative correlation is established between the light intensity stack and the three-dimensional refractive index distribution of the object. The three-dimensional phase transfer function is optimized using circular matching illumination, and the three-dimensional refractive index distribution of cells is reconstructed from the recorded intensity image using the relevant four-dimensional optical transfer function deconvolution algorithm.

Using the internal refractive index of cells as the "endogenous dye", a quantitative correlation is established between the light intensity stack and the three-dimensional refractive index distribution of the object. The three-dimensional phase transfer function is optimized using circular matching illumination, and the three-dimensional refractive index distribution of cells is reconstructed from the recorded intensity image using the relevant four-dimensional optical transfer function deconvolution algorithm.

Using the internal refractive index of cells as the "endogenous dye", a quantitative correlation is established between the light intensity stack and the three-dimensional refractive index distribution of the object. The three-dimensional phase transfer function is optimized using circular matching illumination, and the three-dimensional refractive index distribution of cells is reconstructed from the recorded intensity image using the relevant four-dimensional optical transfer function deconvolution algorithm.

What does IDT image look like?

By combining all images, a high-resolution 3D holographic tomographic reconstruction can be generated, which can be represented as a 2D maximum intensity projection, as you can see on the image here. In this figure, the refractive index is represented by brightness; Darker areas (such as cellular media and cytoplasm) have a lower refractive index, resulting in darker colors. Brighter areas, such as lipid droplets, have a higher refractive index. This can quantify the dry mass of cells and their organelles. Holographic tomography images can provide a deep understanding of the 3D processes of cells and their organelles, such as subcellular localization and organization of organelle systems, membrane dynamics, intercellular interactions, identification of cell death mechanisms, and many other phenotypic changes.

By combining all images, a high-resolution 3D holographic tomographic reconstruction can be generated, which can be represented as a 2D maximum intensity projection, as you can see on the image here. In this figure, the refractive index is represented by brightness; Darker areas (such as cellular media and cytoplasm) have a lower refractive index, resulting in darker colors. Brighter areas, such as lipid droplets, have a higher refractive index. This can quantify the dry mass of cells and their organelles. Holographic tomography images can provide a deep understanding of the 3D processes of cells and their organelles, such as subcellular localization and organization of organelle systems, membrane dynamics, intercellular interactions, identification of cell death mechanisms, and many other phenotypic changes.

By combining all images, a high-resolution 3D holographic tomographic reconstruction can be generated, which can be represented as a 2D maximum intensity projection, as you can see on the image here. In this figure, the refractive index is represented by brightness; Darker areas (such as cellular media and cytoplasm) have a lower refractive index, resulting in darker colors. Brighter areas, such as lipid droplets, have a higher refractive index. This can quantify the dry mass of cells and their organelles. Holographic tomography images can provide a deep understanding of the 3D processes of cells and their organelles, such as subcellular localization and organization of organelle systems, membrane dynamics, intercellular interactions, identification of cell death mechanisms, and many other phenotypic changes.

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Business Email

业务邮箱

Marketing@zircon-opto.com

Business Contact (WeChat)

业务联系（微信）

gaoshi_2024

400-0505-988

南京市建邺区嘉陵江东街B4综合体4层

4th Floor, B4 Complex, Jialingjiang East Street, Jianye District, Nanjing

ZIRCON-2024creght.com

Business Email

业务邮箱

Marketing@zircon-opto.com

Business Contact (WeChat)

业务联系（微信）

gaoshi_2024

400-0505-988

南京市建邺区嘉陵江东街B4综合体4层

4th Floor, B4 Complex, Jialingjiang East Street, Jianye District, Nanjing